报告题目：Determination of in vivo regulation kinetics of small non-coding RNA in bacteria

报告人：费静怡（Center for the Physics of Living Cells, Department of Physics, University of Illinois Urbana-Champaign）

时间：4月22日 下午3:00

地点：教八 218

邀请人：孔学谦

摘要：

Small RNAs (sRNAs) play important roles in regulating gene expression through two major steps: target search by base-pairing interactions with the target messenger RNA (mRNA) and downstream execution by modulating translation or the stability of the mRNA. Here we describe a new imaging and analysis platform based on super-resolution fluorescence microscopy, which enabled the first in vivo kinetic measurement of sRNA-mediated gene regulation. Specifically, this platform was used to investigate a sugar-phosphate stress-induced bacterial sRNA that induces the degradation of target mRNAs. The data reveal that the sRNA binds to a primary target mRNA in a reversible and dynamic fashion, and that formation of the sRNA-mRNA complexes is the rate-limiting step, dictating the overall efficiency of regulation in vivo; whereas the downstream co-degradation of sRNA-mRNA complex can kinetically compete with the fast complex disassembly. Examination of a secondary target of this sRNA indicated that differences in the target search kinetics contribute to setting the regulation priority among different target mRNAs. This super-resolution imaging and analysis approach provides a conceptual framework that can be generalized to other sRNA systems and other target search processes.

报告人介绍：

Dr. JingyiFei graduated from University of Science and Technology of China, with a Bachelor’s degree in Physical chemistry in 2005. She received her Ph.D. from Department of Chemistry at Columbia University in 2010. Her doctoral work with Prof. Ruben Gonzalez was focused on studying conformational dynamics of ribosome using single�molecule fluorescence techniques. During her graduate study, she was awarded Kasindorf Fellowship Award and Hammett Award from Columbia University. She then joined the NSF funded Center for the Physics of Living Cells at University of Illinois Urbana�Champaign as a postdoctoral fellow and worked in the Laboratory of Prof. Taekjip Ha, where she has been taking on a new direction and focusing on non�coding RNA�mediated gene regulation at cellular level using super resolution imaging technique. She will soon join University of Chicago as an assistant professor.